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The proteins were purified from cell culture supernatant using a human albumin affinity column, followed by size exclusion chromatography (SEC), which yielded monomeric fractions that migrated on non-reducing sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels according to their molecular weight (MW) (66.5 kDa) (Fig

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8,9,10,11 In therapeutic amounts, acetaminophen has nearly 100% bioavailability, is approximately 20% bound to serum proteins, has a volume of distribution of around 0.85 L/kg, and has an elimination half-life of approximately 2.5 hours